Coding

Part:BBa_K2625001:Design

Designed by: Axel Norberg, Andrea Clausen Lind, Agnes Torell   Group: iGEM18_Chalmers-Gothenburg   (2018-10-09)


Secretion factor alpha pro + MYR1 myrosinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1023
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A Kozak sequence has been added before the secretion factor. A Kex2 site and a spacer have been placed between the secretion factor and the MYR1. Before the alpha pro, alpha pre has been introduced after the Kozak sequence.


Source

Brassica napus

References